ANTI-CODON MANIPULATION
in
t-RNA
| --- -- -- -- -- - * in bracket, no. of repeat bases |
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|
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| t-RNA1 | -U- -T--A--G--C- -misc. bases--GC/AT--GC/AU- - AG/TC- | |
|
t-RNA2 |
-U- -T--A--G--C- -misc. bases--GC/AT--GC/AU- - AG/TC- | |
|
t-RNA3 |
-U- -T--A--G--C- -misc. bases--GC/AT--GC/AU- - AG/TC- | |
| Split into > | ||
| t-RNA1 (sorting is done here) | count frequency of this word [t-RNA1]> > | |
|
t-RNA2 |
count frequency of this word [t-RNA2]> > | |
| t-RNA3 | count frequency of this word [t-RNA3]> > | |
| Sequence of consecutive at least -- | Find the base at position in [t-RNA1] > - ------ | |
| 2 As , 2 Gs, 2 Us, |
|
Find the base at position in [t-RNA2] > - click- |
| click, click, click, | Find the base at position in [t-RNA3] > - ------- | |
| Mention if CGC followed by TGC click | Slice in between positions in t-RNA1 -- to -->click | |
| Mention if CGC not followed by TGC click | Slice in between positions in t-RNA2 -- to | |
| Slice in between positions in t-RNA3 -- to | ||
| Reverse t-RNA1 | Find last position of word in t-RNA1 -- | |
|
Reverse t-RNA2 |
Find last position of word in t-RNA2 -- click | |
|
Reverse t-RNA3 |
Find last position of word in t-RNA3 -- | |
| Replace : by ----> click click* | ||
| [ in t-RNA1, t-RNA2 & t-RNA3 - all 1st field items will be replaced; on keeping 2nd field blank, all 1st field items will be deleted] | ||
| * only 1st instance of the occurrence in the sequence is replaced, others are left intact. | ||